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KMID : 0379119880160030188
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Korean Journal of Mycology
1988 Volume.16 No. 3 p.188 ~ p.189
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Construction of yeast strain secreting both glucoamylase and α-amylase
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Kim Keun
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Abstract
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Transformed, hybrid strains of the yeast Saccharomyces capable of simultaneous secretion of both glucoamylase and α-amylase have been produced. These strains can carry out direct, one-step assimuilation of starch with conversion efficiency greater than 93% during a 5-day growth period. One of the transformants converts 92.8% of available starch into reducing sugars in only 2 days. Glucoamylase secretion by these strains results from expression of one or more chromosomal STA genes derived from Saccharomyces diastaticus. The strains were transformed by a plasmid (pMS12) containing yeast alcohol dehydrogenase promoter and a segment of yeast 2u plasmid. The major starch hydrolysis product produced by crude amylases found in culture broths is glucose, indicating that α-amylase and glucoamylase act cooperatively.
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